Cryo-TEM: TFS Tundra
The TFS Tundra is a 100 kV cryo-TEM that is capable of intermediate-level resolution of 3-5 Å. The high-brightness field emission gun (X-FEG) paired with the Falcon C Direct Electron Detector increases the resolution of macromolecules to help resolve structures in single particle analysis using the EPU software. This cryo-TEM is ideal for new microscopists and has a user-friendly interface to make automated acquisition accessible while giving biologically relevant data. To insert samples, the Transfer Device on the cryo-loading workstation is used for Autogrid exchange from the cassette to the Tundra. Experienced users can expect to screen up to 10 samples a day with a loading/unloading cycle of approximately 20 minutes.
Hourly rate for academic/non-profit trained users: 70 dollars per hour
Hourly rate for staff imaging of submitted samples from academic/non-profit users: 150 dollars per hour
Hourly rate for staff imaging of submitted samples from for profit users: 300 dollars per hour
A four hour block is billed at 200 (unassisted) and 400 (assisted) for USG/core alliance members
A 24 hour block is billed at 600 (unassisted) and 800 (assisted) for USG/core alliance members
Cryo-Grid prep Assisted: 40 dollars per sample
Cryo-Grid prep Unassisted: 28 dollars per sample
C-Clip and C ring: 23 dollars
Quantifoil Grid: 12 dollars